ࡱ>  ' !"#$%&*()+,-Root Entry F %}3SummaryInformation(DocumentSummaryInformation8XWordDocument34  Oh+'0 , 8 D P \hpx -NVߘTyf[b/gf[O NormalCIFST8@9YO@8@$}3@6+Microsoft Office Word ՜.+,D՜.+,\   K 0t|KSOProductBuildVerICV2052-11.1.0.11365!D2FBC6159C48489D8E248F6E3295B2100TableData WpsCustomData PKSKS34Ihh8p<T " $% h J H`  ,{ASNJ\vũNeP^VExO eXdSf:y& (\5wi) zz1L (\5ў)sQ.͋ 9hǏ'lSirvHRP ؚS Q5u:WPEF & (\5wi) (zz2L) Efficiency of High Pulsed Electric Field on the Activity and conformational change of horseradish Peroxidase(5ўTimes New Roman) (zz1L) Kui Zhong Xiaojun Liao Fenqi Gui Xiaosong Hu(\5 Times New Roman) Food Science and Nutritional Engineering College China Agricultural University Beijing 100083 (\5 Times New Roman) (zz1L) (\5ўTimes New Roman)Abstract Efficiency of high pulsed electric field PEF on the Activity and conformational change of horseradish peroxidase hrp were studied& (\5 Times New Roman) (\5ўTimes New Roman)Key words Horseradish peroxidase HRP Pulsed electric field PEF Circular dichroism CD Fluorescence spectroscopy(\5Times New Roman) N N:N_kXy ,{N\OOo`Y TKb:g{\OOo`Y TKb:g{O_e;NvũW@xxvzN[;NeP^2. vũhQlĉNNNSb/g3. SuߘT0vuCQ0TuCQI{4.vQNebaT Y y %NS %6eU_XdƖ % N6eU_XdƖ ( _4 PAGE   PAGE 2    02HPR\^   ſypgcZVPG>B*phfCJo(B*phCJo( CJPJo(PJo(B*phCJo(PJo(B*phCJo(B*phCJo( CJ PJo(B*phCJo( CJ PJo(B*phCJo(B*phCJo(B*phCJ PJo(B*phCJo(B*phCJ PJo( CJ$PJo( CJ$PJo( CJ$PJo( CJ$PJo(CJ$PJo(mH sH nHtH CJ$PJo(CJ$PJo(mH sH nHtH CJ$PJo( $ & 0 < p z     x z Ĵ{rib[VRMID 5o(\5\ 5o(\5\ 5o(\ CJo(5\ CJo(5\B*phCJo(B*phCJo(B*phfCJo(B*phCJo( CJPJo( CJPJo(B*phCJo(B*phfPJo(B*phCJo(B*phfCJfH q B*phCJo( CJPJo(CJPJ CJPJo( CJPJo(B*phCJo(B*phCJo(B*phCJo(   $ & 8 < T V X p b j ¹}rja]ZVSOI CJo(aJCJo(CJCJo(CJCJo(CJo(aJ5\CJaJ5\B*phCJPJo(B*phCJo(B*phCJo(B*phCJPJB*phCJPJo( CJ6] CJo(6]B*phfCJo(B*phCJo(CJo(CJCJo(CJCJo(CJB*phCJo(B*phCJo(B*phfCJo(B*phCJo(5\ DJLVXt4Z\hjv¹|wqf[RGA CJPJo(B*phCJPJo(B*phCJo(B*phCJPJo(B*phCJPJo( CJo(aJCJaJ CJo(aJCJaJ CJo(aJCJaJ CJo(aJCJaJ CJo(aJCJaJCJo(aJ5\CJaJ5\CJo(aJ5\CJaJ5\B*phCJPJo(B*phCJPJo(B*phCJPJo(B*phfCJPJo(CJo(CJaJvz~ ý}ysog[WOPJo(nHtHPJo(PJo(mH sH nHtHPJo(nHtHPJo( CJPJo(PJo(PJo( CJPJo( CJPJo( CJPJo( CJPJo( CJPJo( CJPJo( CJPJo( CJPJo( CJPJo( CJPJo( CJPJo( CJPJo( CJPJo( CJPJo( CJPJo( CJPJo( CJPJo( CJPJo( CJPJo( CJPJo( CJPJo(.08<HJTVbdprý~tcYSMKHFC0JU0JU CJPJo( CJPJo(CJOJ^Jo(KH!CJOJ^Jo(KH jnHtHCJOJ^Jo(KHCJOJ^JKHCJOJ^Jo(KHCJOJ^JKHCJOJ^Jo(KHCJOJ^JKHCJOJ^Jo(KH CJPJo( CJPJo( CJPJo( CJPJo(PJo(mH sH nHtHPJo(mH sH nHtHPJo(nHtHPJo(PJo(mH sH nHtH CJPJo(o( 0JCJo( 0JCJo(CJU0JCJmHsHnHtHCJU0JCJCJU 0JCJo(  2R^ &   z  & a$$a$$a$$d^WD.`.a$$a$$a$$a$$a$$a$$a$$d4a$$d4a$$ \jxzRF WD`$Ifo$$If:V TT44l44l05$ WD`$IfWD` a$$WD`dh`ha$$dVD^WDd`J>2 WD`$If WD`$If$$If:V TT44l44l0F:    5*5|5~ WD`$If WD`$IfVJ WD`$If$$If:V TT44l44l0F:    5*5|5~ WD`$Ifxl WD`$If WD`$If WD`$Ifo$$If:V TT44l44l05$bSD5a$$WD`$Ifa$$WD`$Ifa$$WD`$If$$If:V TT44l44l0F:    5*5|5~bI:a$$WD`$Ifd1$-DM UD?]?$If$$If:V TT44l44l0F:    5*5|5~:<%$$If:V TT44l44l0F>     5*55z: & F 8dXa$$A$G$ 88$7$1$$$$9D4$5$6$3$H$-DM C$`$Ifxx 8.x&66666666 0@P`p6666 0@P`p 0@P`p 0@P`p 0@P`p 0@P`p 0@P`pJ@Jcke a$$1$ CJaJKHmH sH nHtH_H$A@$؞k=W[SONi@Nnfh@Lha$$@&<CJ OJQJ^JaJ 5\HOaHh Char"0J CJ OJQJ^JaJ 5KH\\@s\Q*:O:ztadd10J B* phW'CJaJ v  <eG.[x @Times New Roman-([SO;4 wiSO_GB23127$B Cambria-4 fN- |8ўSO;4 N[_GB2312?Wingdings 2 -NVߘTyf[b/gf[O CIFST QhgKgɓaf!),.:;?]}    & 6"0000 0 0 00000 =@\]^([{  0 0 00000;[niP)?+}2 CCCCM0]>Y1 GD_:<cgy6(ar@ JKF;f@Bb D  1 >6 d jP p &csbv i,0b3;@PcCg#&co%o;[ R`}t'9NYs':568uN oPu_lp^J/PDj s w &!ez! "L("#G#=f#W@$c_$o$o"&-&c&6y&(>(I*n**(+\+|+',?g,i,(--/_b/"0%0&0ml0"1=1S1Y1^2[2A3GN3c34j4AD5h6v 747eF7g828U98U:8u8E9n9::.:n><|==b=v=/>qZ?<JVLDd4a$$$$If:V TT44l44l005*5a$$WD`$Ifa$$WD`$Ifa$$WD`$Ifd4a$$ 9r 9r h]h 9r #$&` 9r  9r 9r h]h 9r #$&` 9r 1. A!#n"$7%S2P18K@3H@A&BZCMDE8E9@EKF5F:FOmFlrFeGVHjH5IdIJ$J_L/M=MNNPO=YPePQQLQTQQSQ{g{^|{}}#'}+}{~n~z~3# '=BS6:v 6%PdRd-F9%Hd7%AZ7*9 + L}qEmG]6B MK\EnYvE>dX$!yDZibc%P8+)0kU kuu#:k{|9rw3vJ)RSCcen/=R<T\1 #\5GVJRNj )Jw<VF`gpj C.uXtZjsD\_e:"_UaObQyyF=MJo'TTN:M$OKX)f8 (166,m_-E6.RmEbTz8F9Pr~uH=pM|R?u:lQ V#_GjauM@/knpM@/m&+WD.H[\JM}MyQSxXqyd}e4 0(z0(  * 3 ? e!!@