ࡱ> & ' !"#$%+()*,-.Root Entry F%@SummaryInformation(DocumentSummaryInformation8WordDocument.6  Oh+'0 ( 4 @ L Xdlt| -NVߘTyf[b/gf[O NormalW_l8@@UOO@8@߉%@6+Microsoft Office Word ՜.+,D՜.+,\   K (\dlKSOProductBuildVer2052-11.1.0.95840TablenData WpsCustomData PKSKS.6^CW8<0\J"l' $ hy FK  ,{ASNJ\vũNeP^VExO eXdSf:y& (\5wi) zz1L (\5ў)sQ.͋ 9hǏ'lSirvHRP ؚS Q5u:WPEF & (\5wi) (zz2L) Efficiency of High Pulsed Electric Field on the Activity and conformational change of horseradish Peroxidase(5ўTimes New Roman) (zz1L) Kui Zhong Xiaojun Liao Fenqi Gui Xiaosong Hu(\5 Times New Roman) Food Science and Nutritional Engineering College China Agricultural University Beijing 100083 (\5 Times New Roman) (zz1L) (\5ўTimes New Roman)Abstract Efficiency of high pulsed electric field PEF on the Activity and conformational change of horseradish peroxidase hrp were studied& (\5 Times New Roman) (\5ўTimes New Roman)Key words Horseradish peroxidase HRP Pulsed electric field PEF Circular dichroism CD Fluorescence spectroscopy(\5Times New Roman) N N:N_kXy ,{N\OOo`Y TKb:g{\OOo`Y TKb:g{O_e;N1.vũW@xxvz 2.vũN[;NeP^ 3.vũeb/g0eNT 4.vũvhQlĉ 5. vQNebaT Y y %NS %6eU_XdƖ % N6eU_XdƖ % _4 PAGE   PAGE 2    02HPR\^   ſypgcZVPG>B*phfCJo(B*phCJo( CJPJo(PJo(B*phCJo(PJo(B*phCJo(B*phCJo( CJ PJo(B*phCJo( CJ PJo(B*phCJo(B*phCJo(B*phCJ PJo(B*phCJo(B*phCJ PJo( CJ$PJo( CJ$PJo( CJ$PJo( CJ$PJo(CJ$PJo(mH sH nHtH CJ$PJo(CJ$PJo(mH sH nHtH CJ$PJo( $ & 0 < p z     x z Ĵ{rib[VRMID 5o(\5\ 5o(\5\ 5o(\ CJo(5\ CJo(5\B*phCJo(B*phCJo(B*phfCJo(B*phCJo( CJPJo( CJPJo(B*phCJo(B*phfPJo(B*phCJo(B*phfCJfH q B*phCJo( CJPJo(CJPJ CJPJo( CJPJo(B*phCJo(B*phCJo(B*phCJo(   $ & 8 < T V X p b j ¹}rja]ZVSOI CJo(aJCJo(CJCJo(CJCJo(CJo(aJ5\CJaJ5\B*phCJPJo(B*phCJo(B*phCJo(B*phCJPJB*phCJPJo( CJ6] CJo(6]B*phfCJo(B*phCJo(CJo(CJCJo(CJCJo(CJB*phCJo(B*phCJo(B*phfCJo(B*phCJo(5\ DJLVXt4Z\hjv¹|wqf[RGA CJPJo(B*phCJPJo(B*phCJo(B*phCJPJo(B*phCJPJo( CJo(aJCJaJ CJo(aJCJaJ CJo(aJCJaJ CJo(aJCJaJ CJo(aJCJaJCJo(aJ5\CJaJ5\CJo(aJ5\CJaJ5\B*phCJPJo(B*phCJPJo(B*phCJPJo(B*phfCJPJo(CJo(CJaJvz~*,24:ý}ysolh\XTPPJo(PJo(PJo(PJo(mH sH nHtHPJo(PJPJo( CJPJo(PJo(PJo( CJPJo( CJPJo( CJPJo( CJPJo( CJPJo( CJPJo( CJPJo( CJPJo( CJPJo( CJPJo( CJPJo( CJPJo( CJPJo( CJPJo( CJPJo( CJPJo( CJPJo( CJPJo( CJPJo( CJPJo( CJPJo(:bfrt~ż~tnhfca^WSNJUCJ0JCJCJU 0JCJo(0JU0JU CJPJo( CJPJo(CJOJo(^JKHCJOJo(^JKHnHtHCJOJo(^JKHCJOJ^JKHCJOJo(^JKHCJOJ^JKHCJOJo(^JKHCJOJ^JKHCJOJo(^JKH CJPJo( CJPJo( CJPJo( CJPJo(PJo(mH sH nHtHPJo(mH sH nHtH CJPJo(o( 0JCJo( 0JCJo(CJU0JCJmHsHnHtH 2R^ &   z  & a$$a$$a$$d^WD.`.a$$a$$a$$a$$a$$a$$a$$d4a$$d4a$$ \jxzMs$$If:V TT44l44l05$ WD`$IfWD` a$$WD`dh`ha$$dVD^WDd`z9- WD`$If$$If:V TT44l44l0F:    5*5|5~ WD`$If WD`$If WD`$IfE9 WD`$If$$If:V TT44l44l0F:    5*5|5~ WD`$If WD`$Ifsg WD`$If WD`$If WD`$Ifs$$If:V TT44l44l05$]N?0a$$WD`$Ifa$$WD`$Ifa$$WD`$If$$If:V TT44l44l0F:    5*5|5~]D5a$$WD`$Ifd1$-DM UD?]?$If$$If:V TT44l44l0F:    5*5|5~4dda$$1$-DM 0`0$Ifda$$1$-DM 0`0$Ifda$$1$-DM 0`0$If&66666666 0@P`p6666 0@P`p 0@P`p 0@P`p 0@P`p 0@P`p 0@P`pJ@Jcke a$$1$ CJaJKHmH sH nHtH_H$A@$؞k=W[SONi@Nnfh*)@!uxDO1Dh CharCJ OJQJ^JaJ 5KH\L>@Lha$$@&<CJ OJQJ^JaJ 5\@S@R@ckee,g)ۏ 3WD0`0CJPJN@bNu w'a$$G$&dP 9r CJaJ< @r<ua$$G$ 9r CJaJ.@.yblFhe,gCJaJHC@Hckee,g)ۏdHa$$WDX`XCJPJ\@\QY1 GD_:<cgy6(ar@ JKF;f@Bb D  1 >6 d jP p &csbv i,0b3;@PcCg#&co%o;[ R`}t'9NYs':568uN oPu_lp^J/PDj s w &!ez! "L("#G#=f#W@$c_$o$o"&-&c&6y&(>(I*n**(+\+|+',?g,i,(--/_b/"0%0&0ml0"1=1S1Y1^2[2A3GN3c34j4AD5h6v 747eF7g828U98U:8u8E9n9::.:n><|==b=v=/>qZ?K@3H@A&BZCMDE8E9@EKF5F:FOmFlrFeGVHjH5IdIJ$J_L/M=MNdft]N?0a$$WD`$Ifa$$WD`$Ifa$$WD`$If$$If:V TT44l44l0F>     5*55ztlYIG 9r h]h 9r  9r 9r &`#$d4a$$$$If:V TT44l44l005*5d4a$$ 9r h]h 9r  9r 9r &`#$,. A!#n"$7%S2P18NPO=YPePQQLQTQQSQ{g{^|{}}#'}+}{~n~z~3# '=BS6:v 6%PdRd-F9%Hd7%AZ7*9 + L}qEmG]6B MK\EnYvE>dX$!yDZibc%P8+)0kU kuu#:k{|9rw3vJ)RSCcen/=R<T\1 #\5GVJRNj )Jw<VF`gpj C.uXtZjsD\_e:"_UaObQyyF=MJo'TTN:M$OKX)f8 (166,m_-E6.RmEbTz8F9Pr~uH=pM|R?u:lQ V#_GjauM@/knp/WD.H[\JM}Mz6 0(z0(  * 3 ? z!!@